Introduction. Body composition, anthropometry and physical fitness are considered the most important indicators of health assessment, and are widely implemented in different sports. Aim of Study. The aim of this study was to examine relationships between body composition, anthropometry and physical fitness of female students of the Khorramshahr University of Marine Science and Technology in Iran. Material and Methods. The study sample comprised 156 randomly selected female university students attending a general physical education course at the Khorramshahr University of Marine Science and Technology in the years of 2015-2016. The AAHPERD test was used for physical fitness assessment. Data were analyzed using the SPSS software, descriptive statistical methods and Pearson’s correlation coefficient at the level of statistical significance of p ≤ 0.05. Results. The results showed a significant correlation between physical fitness, body composition and anthropometry in the female students (p > 0.05). The statistical analysis confirmed their mean physical fitness level of 16.85 ± 1.9. Conclusions. The results of the study revealed a statistically significant relationship between physical fitness, body composition and anthropometry in female students. Also, the study showed that regular exercise improved physical health. It is suggested that more attention should be paid to sport programs as well as to the development of university students’ physical fitness and general health. It is recommended that students should participate in health promoting activities. University authorities should be expected to provide their institutions with sport and welfare facilities