Preschool-aged children respond very positively to the educational impact based on targeted incentives and suitable designs. It is thus relatively easy to cultivate the habit of healthy lifestyle choices and optimal physical exercise regimen at this stage of life. The family plays a fundamental role in forming a healthy lifestyle of children. The aim of the present study was to analyse relationships between family’s socio-economic status and the physical activity levels of preschool-aged children. The research sample consisted of 200 children (96 girls, 104 boys) aged 5.71 ± 1.0 years. Family history (parents’ age; parents’ education; number of siblings; type of housing; economic, spatial and traffic stimuli; participation in physical activity) was assessed with the use of a modified Czech version of the Environmental Stimulus for Physical Activities questionnaire[1]. Informed consent was obtained from the parents before any research procedures. The level of physical activity was evaluated on the basis of the results of monitoring active energy expenditure (kcal × kg × day–1) recorded with ActiGraphGT3X+. The parents’ education was found to be related to the children’s greater involvement in sports organizations (p < 0.03).The statistically significant relationship noted between the level of parents’ education and economic and spatial stimuli at home (p < 0.01) confirmed the purposeful and intentional influence of parents in the optimization of daily exercise regimen of their children. The parents’ inclination towards the development of their children’s active lifestyle is closely related to the children’s membership and participation in youth/sports organizations (p < 0.002)