Introduction. In modern sport, training individualization anddetailed analysis of specific patterns of biochemical indices undercertain exercises and training sessions is become more and morecrucial. Aim of Study. This study aimed to concurrently evaluatethe lactate and blood ammonia response during resistance trainingin four elite sprinters (two men and two women). Material andMethods. Blood samples were taken from the fingertip before andafter the warm-up, after each exercise (power cleans, squat jumps,quarter squats and lunges), and at the 10th and 20th min of the cooldown.Results. In male athletes, maximum lactate concentrationswere achieved after the power clean exercise, while peak bloodammonia concentrations after squat jumps. In female athletes,peak blood ammonia and lactate concentrations were noted moreindividually. The course of changes in lactate concentrationswas very diverse in each athlete. The ammonia concentration inresponse to the performed exercises was much more consistent,however still different between individual athletes. Conclusions.A practical analysis of the metabolic response to different exercisesin a resistance training session, using lactate and ammoniaconcentrations, offers vital information that can help coachesbetter understand internal training load experienced by theathlete and to better adjust the prescribed loads and rest periodsto the training targets in future training sessions.