Introduction. Objective analysis of movement and position is useful for prevention of strains and injuries in professional sport. Correct bike fitting can be performed on the basis of a motion analysis system. Aim of Study. The aim of the study was to analyze changes in the position of female cyclists before and after bike fitting. Material and Methods. The study sample consisted of four female cyclists from a professional Polish cycling team TKK Pacific. The cyclists’ position on the bike was analyzed with an optoelectronic system BTS SMART DX 7000, before and after bike fitting. Results. The statistical analysis did not reveal any differences in the cyclists’ position before and after bike fitting. Individual results of particular participants were analyzed and suggestions related to changes in the position on the bike were given. All the studied cyclists still needed adjustment of their trunk and ankle joint positions. Three participants should have had a correction of the elbow joint angle. One cyclist needed a correction of the knee joint. The hip joint position was correct in all the cyclists. Conclusions. Cyclists should have their bikes fitted in a detailed and objective way. Objective movement analysis based on optoelectronic measurement as well as individual approach to each professional cyclist can improve the cyclist’s position on the bike and minimize the risk of injuries