ORIGINAL ARTICLE
Immediate and short-term effects of warm-up, dynamic
and static stretching on hamstring flexibility in college
students with muscle tightness
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1
Saveetha College of Physiotherapy, Saveetha Institute of
Medical and Technical Sciences, India
2
Department of Obstetrics and Gynaecology, School of
Physiotherapy, Dhanalakshmi Srinivasan University, India
3
Department of Orthopaedics, School of Physiotherapy,
Dhanalakshmi Srinivasan University, India
4
Rathinam College of Physiotherapy, Rathinam Group of
Institutions, India
Submission date: 2025-08-01
Final revision date: 2025-11-26
Acceptance date: 2025-12-01
Publication date: 2026-06-30
Corresponding author
Anitha Arul
Associate Professor, Saveetha College of Physiotherapy, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamilnadu, India., India
TRENDS in Sport Sciences 2026;33(2)
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ABSTRACT
Introduction:
Frequently observed in athletes, hamstring
muscle tightness impairs their performance and increases injury
risk. While static and dynamic stretching are commonly used to
improve flexibility, the comparative effectiveness and short-term
retention of gains following these interventions remain unclear.
Aim of the study:
This study aimed to investigate the immediate and
short-term effects of warm-up, static and dynamic stretching on
hamstring flexibility in college students.
Material and methods:
crossover study was carried out among forty college students
with clinically confirmed hamstring tightness. The subjects
underwent three interventions - warm-up, static stretching, and
dynamic stretching in random order. Hamstrings flexibility was
evaluated with passive knee extension range of motion (PKE
ROM) test.
Results:
two-way repeated measures ANOVA
examined the effects of static vs. dynamic stretching and time. The
average ROM across all time points did not differ significantly
between the two stretches [F(1,312) = 0.291, p = 0.590, partial
η² = 0.0009] and produced comparable overall ROM values.
ROM changes across the 4 time points approached significance
[F(3,312) = 2.275, p = 0.080, partial η² = 0.0214], indicating small
but observable variations across the measurement timeline.
The Condition × Time interaction was not significant [F(3,312)
= 0.767, p = 0.513, partial η² = 0.0073], demonstrating that the
pattern of ROM change over time was similar for both stretches.
Effect sizes were estimated using Cohen’s d, which revealed
greater within-condition ROM improvement following static
stretching, while post-stretch differences between stretching
types were small. Warm-up alone accounted for approximately
3.5° and 5.8° of ROM improvement. Static stretching produced
a larger additional increase than dynamic stretching, while
both conditions showed high short-term retention.
Conclusions:
Warm-up, static and dynamic stretching techniques are effective
in improving hamstring flexibility in the short term. However,
flexibility gains may diminish slightly after 15 minutes.