Introduction:
Interleukin 10 (IL-10) is a cytokine with strong anti-inflammatory properties that plays an important role when produced during exercise, and has a role in limiting the body’s immune response to pathogens and preventing tissue damage.

Aim of the study:
This study aimed to investigate the effect of highintensity ergocycle training (HIET) on increasing IL-10 levels in healthy adolescent males.

Material and methods:
A trueexperimental design with pretest–posttest control groups was employed. The study involved 30 adolescent males aged 19–24 years, with a body mass index (BMI) of 19-22 kg/m2 and no history of chronic disease. The participants were randomly assigned to two groups: G1 (n = 15, control group) and G2 (n = 15, HIET group). The HIET regimen involved pedaling an ergocycle for 40-60 minutes, including a 5-minute warm-up at 50-60% maximum heart rate (HRmax), 30-50 minutes of interval training at 80-90% HRmax (6 sets of 5 minutes each, with 2 minutes of active rest between sets), and a 5-minute cooldown at 50-60% HRmax. This intervention was conducted over the period of 4 weeks, with sessions held three times per week. IL-10 levels were measured via enzyme-linked immunosorbent assay (ELISA), using an ELISA kit. Statistical analysis was performed via the independent samples t-test with a significance level of 5%.

Results:
The results revealed no significant difference in pre-HIET IL-10 levels between G1 and G2 (7.64 ± 1.87 vs 8.41 ± 1.60 pg/mL; p = 0.239; ES: 0.442). However, post-HIET IL-10 levels were significantly greater in G2 than in G1 (8.33 ± 1.57 vs 14.35 ± 3.33 pg/mL; p = 0.001; ES: 2.312), and the change in IL-10 levels (delta) was also significantly greater in G2 (0.68 ± 0.48 vs 5.94 ± 0.68 pg/mL; p = 0.001; ES: 8.937).

Conclusions:
These findings indicate that HIET effectively enhances the antiinflammatory response, as evidenced by increased IL-10 levels in healthy adolescent males.